Summary: MADE4, microarray ade4, is a software package that facilitates multivariate analysis of microarray gene expression data. MADE4 accepts a wide variety of gene expression data formats. MADE4 takes advantage of the extensive multivariate statistical and graphical functions in the R package ade4, extending these for application to microarray data. In addition MADE4 provides new graphical and visualisation tools that aid in interpretation of multivariate analysis of microarray data. Availability: The R package MADE4 is available from Bioconductor www.bioconductor.org and bioinf.ucd.ie/software/. Supplementary information: MADE4 is well documented. There are tutorials, in the form of vignettes, which describe typical analyses. In addition the MADE4 manual provides descriptions and examples for each function.